Pi
0.00395
MarkerSeek score
54.90
Primer available
yes
Label
rps15-trnN-GUU
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rps15-trnN-GUU_p1 | TCAGGAGACAATAGACTTTGACA | TCGATATCTATGGACCATCGA | 39.1 | 42.9 | 56.9 | 55.3 | 303 bp | 1.0 | 41.1 |
| rps15-trnN-GUU_p2 | TCAGGAGACAATAGACTTTGACA | TCGATATCTATGGACCATCGAA | 39.1 | 40.9 | 56.9 | 56.1 | 303 bp | 1.0 | 41.1 |
| rps15-trnN-GUU_p3 | CAGGAGACAATAGACTTTGACA | TCGATATCTATGGACCATCGA | 40.9 | 42.9 | 55.5 | 55.3 | 302 bp | 1.0 | 41.1 |
| rps15-trnN-GUU_p4 | TCAGGAGACAATAGACTTTGACA | ATCGATATCTATGGACCATCGA | 39.1 | 40.9 | 56.9 | 55.8 | 304 bp | 1.0 | 41.1 |
| rps15-trnN-GUU_p5 | TCAGGAGACAATAGACTTTGACA | TCGATATCTATGGACCATCGAAA | 39.1 | 39.1 | 56.9 | 56.8 | 303 bp | 1.0 | 41.1 |