Hotspot

ndhF-rpl32

SSC · 109277-109905 · 629 bp

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Pi 0
MarkerSeek score 51.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
629
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CAGATTTTTGGTTGCCGTCG TGAAAAAGCTTTCAATCTTGTCCA 50.0 33.3 58.3 57.8 833-836 bp 1.0 44.0
ndhF-rpl32_p2 CACACGACGCAGATTTTTGG TGAAAAAGCTTTCAATCTTGTCCA 50.0 33.3 58.3 57.8 842-845 bp 1.0 43.9
ndhF-rpl32_p3 ACACACGACGCAGATTTTTG TGAAAAAGCTTTCAATCTTGTCCA 45.0 33.3 57.6 57.8 843-846 bp 1.0 43.5
ndhF-rpl32_p4 CACACACGACGCAGATTTTT TGAAAAAGCTTTCAATCTTGTCCA 45.0 33.3 57.6 57.8 844-847 bp 1.0 43.5
ndhF-rpl32_p5 ACGACGCAGATTTTTGGTTG TGAAAAAGCTTTCAATCTTGTCCA 45.0 33.3 57.6 57.8 839-842 bp 1.0 43.3