Hotspot

rpl16-rps3

LSC · 80248-81565 · 1318 bp

Back to Uraria

Pi 0.00762
MarkerSeek score 63.60
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
1318
variable_sites
10
pi
0.00762
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 CTCAAGTGCCTGAAGAGCAT TCCTCTACAAACCATTCGAGC 50.0 47.6 57.9 57.8 1535-1541 bp 1.0 70.0
rpl16-rps3_p2 CTCAAGTGCCTGAAGAGCAT CCTCTACAAACCATTCGAGCT 50.0 47.6 57.9 57.7 1534-1540 bp 1.0 69.9
rpl16-rps3_p3 CTCAAGTGCCTGAAGAGCAT GGTAGGGTTCCTCTACAAACC 50.0 52.4 57.9 57.4 1543-1549 bp 1.0 66.9
rpl16-rps3_p4 CTCAAGTGCCTGAAGAGCAT AGAGAAGGTAGGGTTCCTCT 50.0 50.0 57.9 56.4 1549-1555 bp 1.0 66.8
rpl16-rps3_p5 CTCAAGTGCCTGAAGAGCAT CCTCTACAAACCATTCGAGC 50.0 50.0 57.9 56.2 1534-1540 bp 1.0 65.9