Hotspot

psaJ-rpl33

LSC · 65363-65860 · 498 bp

Back to Uraria

Pi 0.012
MarkerSeek score 49.90
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
498
variable_sites
6
pi
0.012
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 CGAACTGAACTAAGAGCGCT TGGAAGGGTAACACACAAGC 50.0 50.0 58.0 58.0 1389-1411 bp 1.0 81.4
psaJ-rpl33_p2 ACGTTCTGTACATCACGCTC TGGAAGGGTAACACACAAGC 50.0 50.0 58.0 58.0 1457-1479 bp 1.0 81.3
psaJ-rpl33_p3 ACATATCTTTCCGTGGCACC TGGAAGGGTAACACACAAGC 50.0 50.0 58.0 58.0 843-851 bp 1.0 81.0
psaJ-rpl33_p4 GCGTTCTACCGAACTGAACT TGGAAGGGTAACACACAAGC 50.0 50.0 57.9 58.0 1398-1420 bp 1.0 81.0
psaJ-rpl33_p5 CGAACTGAACTAAGAGCGCT AGGGTAACACACAAGCGATC 50.0 50.0 58.0 57.9 1385-1407 bp 1.0 80.8