Hotspot

psbE-petL

LSC · 62784-64116 · 1333 bp

Back to Uraria

Pi 0.00305
MarkerSeek score 62.20
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1333
variable_sites
4
pi
0.00305
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC GACCAATAAATAGAGCGGAGGT 50.0 45.5 57.8 57.7 1419-1433 bp 1.0 61.6
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC GACCAATAAATAGAGCGGAGGT 55.0 45.5 58.2 57.7 1414-1428 bp 1.0 61.2
psbE-petL_p3 CCTGCAATGAATAGGGAGGG GACCAATAAATAGAGCGGAGGT 55.0 45.5 57.7 57.7 1493-1507 bp 1.0 60.5
psbE-petL_p4 AGAACGTTCTCCTGTGCTTC ACCAATAAATAGAGCGGAGGT 50.0 42.9 57.8 56.5 1418-1432 bp 1.0 60.3
psbE-petL_p5 TCTCCTGTGCTTCCAGACAT GACCAATAAATAGAGCGGAGGT 50.0 45.5 58.4 57.7 1412-1426 bp 1.0 60.0