Hotspot

trnR-UCU-trnS-GCU

LSC · 51618-52919 · 1302 bp

Back to Uraria

Pi 0.00699
MarkerSeek score 62.20
Primer available yes
Label trnR-UCU-trnS-GCU

Feature Metadata

label_name
trnR-UCU-trnS-GCU
feature_type
igs
region
LSC
length_bp
1302
variable_sites
9
pi
0.00699
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnS-GCU_p1 AGAAGACCTCTGTCCTATCCA CAATCCGACGCTTTAGTCCA 47.6 50.0 56.9 58.0 1361-1373 bp 1.0 64.9
trnR-UCU-trnS-GCU_p2 AGAAGACCTCTGTCCTATCCA AATCCGACGCTTTAGTCCAC 47.6 50.0 56.9 58.0 1360-1372 bp 1.0 64.9
trnR-UCU-trnS-GCU_p3 AGGTTTAGAAGACCTCTGTCCT CAATCCGACGCTTTAGTCCA 45.5 50.0 57.9 58.0 1367-1379 bp 1.0 64.2
trnR-UCU-trnS-GCU_p4 AGGTTTAGAAGACCTCTGTCCT AATCCGACGCTTTAGTCCAC 45.5 50.0 57.9 58.0 1366-1378 bp 1.0 64.2
trnR-UCU-trnS-GCU_p5 AGAAGACCTCTGTCCTATCCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 56.9 58.2 1414-1426 bp 1.0 63.7