Hotspot

ccsA-psaC

SSC · 116619-118486 · 1868 bp

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Pi 0.00943

MarkerSeek score 52.60

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: SSC
length_bp: 1868
variable_sites: 54
pi: 0.00943
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	TAGGAATGGGGCTGCATAGT	GCCTGTCCAACGGATTTCTT	50.0	50.0	58.2	58.5	1988-2000 bp	1.0	77.4
ccsA-psaC_p2	GGGGCTGCATAGTTATGGTT	GCCTGTCCAACGGATTTCTT	50.0	50.0	57.6	58.5	1981-1993 bp	1.0	75.9
ccsA-psaC_p3	TCAGCAATTGTAGCGTCTGT	GCCTGTCCAACGGATTTCTT	45.0	50.0	57.5	58.5	2052-2064 bp	1.0	75.1
ccsA-psaC_p4	TAGGAATGGGGCTGCATAGT	ATGGCATGAAACAACTCGCA	50.0	45.0	58.2	58.8	1950-1962 bp	1.0	75.1
ccsA-psaC_p5	ATGGGGCTGCATAGTTATGG	GCCTGTCCAACGGATTTCTT	50.0	50.0	57.4	58.5	1983-1995 bp	1.0	74.1