Hotspot

ndhF-rpl32

SSC · 113660-114469 · 810 bp

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Pi 0.0162

MarkerSeek score 61.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 810
variable_sites: 40
pi: 0.0162
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGAAAGCGGAATGAAGGGT	TGATGTAGAAGTGCGCTTTT	45.0	40.0	57.7	55.7	835-910 bp	1.0	66.8
ndhF-rpl32_p2	TTGAAAGCGGAATGAAGGGT	TTGATGTAGAAGTGCGCTTT	45.0	40.0	57.7	55.7	836-911 bp	1.0	66.8
ndhF-rpl32_p3	TTGAAAGCGGAATGAAGGGT	TTTGATGTAGAAGTGCGCTT	45.0	40.0	57.7	55.7	837-912 bp	1.0	66.8
ndhF-rpl32_p4	TTGAAAGCGGAATGAAGGGT	TTTTGATGTAGAAGTGCGCT	45.0	40.0	57.7	55.7	838-913 bp	1.0	66.8
ndhF-rpl32_p5	TTGAAAGCGGAATGAAGGGT	TGATGTAGAAGTGCGCTTTTT	45.0	38.1	57.7	56.5	835-910 bp	1.0	65.0