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psbE-petL

LSC · 66448-67532 · 1085 bp

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Pi 0.0128

MarkerSeek score 60.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1085
variable_sites: 37
pi: 0.0128
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGTCGTATCTTGCTCAAACCA	50.0	42.9	57.9	57.3	1266-1309 bp	1.0	69.5
psbE-petL_p2	ATAGCCAACCCGCAATGAAT	AGTCGTATCTTGCTCAAACCA	45.0	42.9	57.9	57.3	1271-1314 bp	1.0	69.4
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAAACCA	50.0	42.9	57.8	57.3	1189-1232 bp	1.0	68.9
psbE-petL_p4	AATAGCCAACCCGCAATGAA	AGTCGTATCTTGCTCAAACCA	45.0	42.9	58.2	57.3	1272-1315 bp	1.0	68.7
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	AGTCGTATCTTGCTCAAACCA	55.0	42.9	58.2	57.3	1184-1227 bp	1.0	68.6