Hotspot

ycf4-cemA

LSC · 61930-62778 · 849 bp

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Pi 0.0111

MarkerSeek score 57.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 25
pi: 0.0111
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ACAAAAAGCTGCCGAATTGG	ATAAAGGGGGAGTGAGTGCT	45.0	50.0	57.8	58.0	908-938 bp	1.0	83.5
ycf4-cemA_p2	TTTGCGCGTACCAATTGAAG	ATAAAGGGGGAGTGAGTGCT	45.0	50.0	57.7	58.0	881-911 bp	1.0	82.3
ycf4-cemA_p3	GCGCGTACCAATTGAAGTTT	ATAAAGGGGGAGTGAGTGCT	45.0	50.0	57.7	58.0	878-908 bp	1.0	82.3
ycf4-cemA_p4	ACAAAAAGCTGCCGAATTGG	TAAAGGGGGAGTGAGTGCTT	45.0	50.0	57.8	58.3	907-937 bp	1.0	81.7
ycf4-cemA_p5	ACAAAAAGCTGCCGAATTGG	GGGGAGTGAGTGCTTTCTTT	45.0	50.0	57.8	57.7	902-932 bp	1.0	81.5