Hotspot

ndhC-trnV-UAC

LSC · 51449-52630 · 1182 bp

Back to Uncaria

Pi 0.0131
MarkerSeek score 59.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1182
variable_sites
43
pi
0.0131
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.90
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTCCGGAAATTAAAAAGGCCA AGAAGGTCTACGGTTCGAGT 42.9 50.0 56.8 57.8 1169-1353 bp 1.0 67.4
ndhC-trnV-UAC_p2 ACTCCGGAAATTAAAAAGGCC AGAAGGTCTACGGTTCGAGT 42.9 50.0 56.8 57.8 1170-1354 bp 1.0 67.3
ndhC-trnV-UAC_p3 TCCGGAAATTAAAAAGGCCA AGAAGGTCTACGGTTCGAGT 40.0 50.0 55.5 57.8 1168-1352 bp 1.0 65.3
ndhC-trnV-UAC_p4 ACTCCGGAAATTAAAAAGGCCA AGAAGGTCTACGGTTCGAGT 40.9 50.0 58.5 57.8 1170-1354 bp 1.0 65.2
ndhC-trnV-UAC_p5 CTCCGGAAATTAAAAAGGCCAA AGAAGGTCTACGGTTCGAGT 40.9 50.0 57.5 57.8 1169-1353 bp 1.0 65.0