Hotspot

trnS-UGA-psbZ

LSC · 36582-36921 · 340 bp

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Pi 0.00746

MarkerSeek score 56.50

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 340
variable_sites: 8
pi: 0.00746
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CTATCAACCACTCAGCCACC	GAAGCAAATACAACGGGCAC	55.0	50.0	58.0	58.0	442-457 bp	1.0	82.8
trnS-UGA-psbZ_p2	CTATCAACCACTCAGCCACC	AATACAACGGGCACGCTAAT	55.0	45.0	58.0	58.0	436-451 bp	1.0	82.7
trnS-UGA-psbZ_p3	CTATCAACCACTCAGCCACC	AGAAGCAAATACAACGGGCA	55.0	45.0	58.0	58.1	443-458 bp	1.0	82.2
trnS-UGA-psbZ_p4	GTTTTCAAGACCGGGGCTAT	GAAGCAAATACAACGGGCAC	50.0	50.0	57.9	58.0	458-473 bp	1.0	81.9
trnS-UGA-psbZ_p5	GTTTTCAAGACCGGGGCTAT	AATACAACGGGCACGCTAAT	50.0	45.0	57.9	58.0	452-467 bp	1.0	81.9