Hotspot

trnT-GGU-psbD

LSC · 32513-33844 · 1332 bp

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Pi 0.00684

MarkerSeek score 59.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1332
variable_sites: 27
pi: 0.00684
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1423-1474 bp	1.0	84.7
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1426-1477 bp	1.0	84.5
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1441-1492 bp	1.0	83.7
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1444-1495 bp	1.0	83.5
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1400-1451 bp	1.0	83.0