Hotspot

psbM-trnD-GUC

LSC · 30393-31284 · 892 bp

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Pi 0.00973

MarkerSeek score 56.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 892
variable_sites: 24
pi: 0.00973
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGAACTAGAATGAATAGTGCAGT	TTCAATTGGTCAGAGCACCG	37.5	50.0	57.4	58.5	1002-1017 bp	1.0	42.7
psbM-trnD-GUC_p2	GGAACTAGAATGAATAGTGCAGT	TTCAATTGGTCAGAGCACCG	39.1	50.0	56.1	58.5	1001-1016 bp	1.0	42.7
psbM-trnD-GUC_p3	CGTAGGAACTAGAATGAATAGTGC	TTCAATTGGTCAGAGCACCG	41.7	50.0	57.0	58.5	1005-1020 bp	1.0	42.7
psbM-trnD-GUC_p4	TTGACTGACTGTTTTTACGT	TTCAATTGGTCAGAGCACCG	35.0	50.0	53.0	58.5	1044-1059 bp	1.0	42.6
psbM-trnD-GUC_p5	TTTGACTGACTGTTTTTACGT	TTCAATTGGTCAGAGCACCG	33.3	50.0	53.9	58.5	1045-1060 bp	1.0	42.6