Hotspot

petN-psbM

LSC · 29245-30287 · 1043 bp

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Pi 0.00661

MarkerSeek score 57.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1043
variable_sites: 25
pi: 0.00661
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	AGTTCCTACGGCTTTTCTACT	50.0	42.9	58.0	56.4	1077-1132 bp	1.0	64.9
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	AGTTCCTACGGCTTTTCTACT	50.0	42.9	58.0	56.4	1075-1130 bp	1.0	64.9
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACGGCT	50.0	45.0	58.0	55.4	1084-1139 bp	1.0	64.7
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACGGCT	50.0	45.0	58.0	55.4	1082-1137 bp	1.0	64.7
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	AGTTCCTACGGCTTTTCTACT	55.0	42.9	57.9	56.4	1074-1129 bp	1.0	64.3