Hotspot

psbK-psbI

LSC · 8023-8443 · 421 bp

Back to Uncaria

Pi 0.014

MarkerSeek score 60.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 18
pi: 0.014
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	CCCTTTATTCTTCACGCCCA	45.0	50.0	58.1	57.9	568-580 bp	1.0	82.2
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	CCCTTTATTCTTCACGCCCA	50.0	50.0	57.9	57.9	563-575 bp	1.0	82.2
psbK-psbI_p3	ATGCCAGTCATACCTGTCCT	CCCTTTATTCTTCACGCCCA	50.0	50.0	58.1	57.9	602-614 bp	1.0	81.5
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	CCCTTTATTCTTCACGCCCA	45.0	50.0	58.3	57.9	560-572 bp	1.0	80.2
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	CCCTTTATTCTTCACGCCCA	45.0	50.0	58.3	57.9	561-573 bp	1.0	80.2