Hotspot

trnK-UUU-rps16

LSC · 4229-5073 · 845 bp

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Pi 0.0127
MarkerSeek score 58.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
845
variable_sites
30
pi
0.0127
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 857-975 bp 1.0 83.7
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 856-974 bp 1.0 83.7
trnK-UUU-rps16_p3 TCCTAGCCGCACTTAAAAGC GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 872-990 bp 1.0 82.8
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 866-984 bp 1.0 82.7
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.5 58.2 843-961 bp 1.0 81.4