Hotspot

trnH-GUG-psbA

LSC · 86-373 · 288 bp

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Pi 0.0491

MarkerSeek score 72.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 288
variable_sites: 36
pi: 0.0491
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	AATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.7	57.9	335-358 bp	1.0	88.7
trnH-GUG-psbA_p2	AATCCACTTGGCTACATCCG	TCCCTCTAGACCTAGCTGCT	50.0	55.0	57.7	58.8	334-357 bp	1.0	83.2
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	CCCTCTAGACCTAGCTGCTA	50.0	55.0	57.7	57.0	333-356 bp	1.0	81.7
trnH-GUG-psbA_p4	TAATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	47.6	55.0	57.5	57.9	336-359 bp	1.0	79.7
trnH-GUG-psbA_p5	AATCCACTTGGCTACATCCG	ACTTCCCTCTAGACCTAGCTG	50.0	52.4	57.7	57.7	337-360 bp	1.0	78.9