Hotspot

rpoB

Genome · 75690-78893 · 3204 bp

Back to Ulota

Pi 0.0042
MarkerSeek score 44.00
Primer available yes
Label rpoB

Feature Metadata

label_name
rpoB
feature_type
gene
region
Genome
length_bp
3204
variable_sites
11
pi
0.0042
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB_p1 GTCCGGCATTCATACCTTCA AGACCTCTGCCTTACCACTT 50.0 50.0 57.7 58.0 1593 bp 0.5 57.1
rpoB_p2 GTCCGGCATTCATACCTTCA TTACCACTTGGCCATATCGC 50.0 50.0 57.7 58.0 1582 bp 0.5 57.1
rpoB_p3 GTCCGGCATTCATACCTTCA GACCTCTGCCTTACCACTTG 50.0 55.0 57.7 57.9 1592 bp 0.5 56.6
rpoB_p4 TGAGTCCGGCATTCATACCT AGACCTCTGCCTTACCACTT 50.0 50.0 58.5 58.0 1596 bp 0.5 55.7
rpoB_p5 TGAGTCCGGCATTCATACCT TTACCACTTGGCCATATCGC 50.0 50.0 58.5 58.0 1585 bp 0.5 55.7