Hotspot

rpoC1-rpoB

Genome · 75666-75689 · 24 bp

Back to Ulota

Pi 0.0833
MarkerSeek score 85.40
Primer available yes
Label rpoC1-rpoB

Feature Metadata

label_name
rpoC1-rpoB
feature_type
igs
region
Genome
length_bp
24
variable_sites
2
pi
0.0833
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1-rpoB_p1 GCCCAACTGCGTATTTGTTC ACTGCTCCAGAATCTTTTCGA 50.0 42.9 58.0 57.3 209 bp 1.0 67.1
rpoC1-rpoB_p2 TTCAGCCCAACTGCGTATTT ACTGCTCCAGAATCTTTTCGA 45.0 42.9 58.1 57.3 213 bp 1.0 66.5
rpoC1-rpoB_p3 TTTCAGCCCAACTGCGTATT ACTGCTCCAGAATCTTTTCGA 45.0 42.9 58.1 57.3 214 bp 1.0 66.5
rpoC1-rpoB_p4 AGCCCAACTGCGTATTTGTT ACTGCTCCAGAATCTTTTCGA 45.0 42.9 58.4 57.3 210 bp 1.0 64.2
rpoC1-rpoB_p5 CTTTCAGCCCAACTGCGTAT ACTGCTCCAGAATCTTTTCGA 50.0 42.9 58.5 57.3 215 bp 1.0 62.9