Hotspot

ycf12-trnG-UCC

Genome · 61963-62096 · 134 bp

Back to Ulota

Pi 0.0821
MarkerSeek score 85.30
Primer available yes
Label ycf12-trnG-UCC

Feature Metadata

label_name
ycf12-trnG-UCC
feature_type
igs
region
Genome
length_bp
134
variable_sites
11
pi
0.0821
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf12-trnG-UCC_p1 ATTAGCTGCACGTAAAGGCA ACGAATCACACTTTTACCACT 45.0 38.1 57.9 55.5 196 bp 1.0 55.3
ycf12-trnG-UCC_p2 TTAGCTGCACGTAAAGGCAA ACGAATCACACTTTTACCACT 45.0 38.1 58.1 55.5 195 bp 1.0 55.3
ycf12-trnG-UCC_p3 ATTAGCTGCACGTAAAGGCA AGAACGAATCACACTTTTACCAC 45.0 39.1 57.9 57.3 199 bp 1.0 54.2
ycf12-trnG-UCC_p4 ATTAGCTGCACGTAAAGGCA GAACGAATCACACTTTTACCACT 45.0 39.1 57.9 57.3 198 bp 1.0 54.2
ycf12-trnG-UCC_p5 TTAGCTGCACGTAAAGGCAA GAACGAATCACACTTTTACCACT 45.0 39.1 58.1 57.3 197 bp 1.0 54.2