Hotspot

trnG-GCC-psbZ

Genome · 41832-42080 · 249 bp

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Pi 0.00803
MarkerSeek score 48.90
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
Genome
length_bp
249
variable_sites
2
pi
0.00803
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 GGGAATCGAACCCGCATATT CTTGCCTCTCCTGATGGTTG 50.0 55.0 58.1 58.3 421 bp 1.0 77.5
trnG-GCC-psbZ_p2 CGAACCCGCATATTCTCCTT CTTGCCTCTCCTGATGGTTG 50.0 55.0 57.8 58.3 415 bp 1.0 76.4
trnG-GCC-psbZ_p3 CCGCATATTCTCCTTGGCAA CTTGCCTCTCCTGATGGTTG 50.0 55.0 58.3 58.3 410 bp 1.0 75.8
trnG-GCC-psbZ_p4 AACCCGCATATTCTCCTTGG CTTGCCTCTCCTGATGGTTG 50.0 55.0 57.7 58.3 413 bp 1.0 75.6
trnG-GCC-psbZ_p5 ATCGAACCCGCATATTCTCC CTTGCCTCTCCTGATGGTTG 50.0 55.0 57.5 58.3 417 bp 1.0 74.7