Hotspot

trnfM-CAU-trnG-GCC

Genome · 41648-41760 · 113 bp

Back to Ulota

Pi 0.0354
MarkerSeek score 63.30
Primer available yes
Label trnfM-CAU-trnG-GCC

Feature Metadata

label_name
trnfM-CAU-trnG-GCC
feature_type
igs
region
Genome
length_bp
113
variable_sites
4
pi
0.0354
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-trnG-GCC_p1 GTAGCTCGCAAGGCTCATAA AATATGCGGGTTCGATTCCC 50.0 50.0 57.8 58.1 196 bp 1.0 79.0
trnfM-CAU-trnG-GCC_p2 TAGCTCGCAAGGCTCATAAC AATATGCGGGTTCGATTCCC 50.0 50.0 57.8 58.1 195 bp 1.0 79.0
trnfM-CAU-trnG-GCC_p3 GTAGCTCGCAAGGCTCATAA AAGGAGAATATGCGGGTTCG 50.0 50.0 57.8 57.8 202 bp 1.0 77.9
trnfM-CAU-trnG-GCC_p4 TAGCTCGCAAGGCTCATAAC AAGGAGAATATGCGGGTTCG 50.0 50.0 57.8 57.8 201 bp 1.0 77.9
trnfM-CAU-trnG-GCC_p5 TAGCTCGCAAGGCTCATAAC TTGCCAAGGAGAATATGCGG 50.0 50.0 57.8 58.3 206 bp 1.0 77.2