Hotspot

psbJ-petA

Genome · 19111-20134 · 1024 bp

Back to Ulota

Pi 0.00489
MarkerSeek score 61.70
Primer available yes
Label psbJ-petA

Feature Metadata

label_name
psbJ-petA
feature_type
igs
region
Genome
length_bp
1024
variable_sites
5
pi
0.00489
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbJ-petA_p1 AGGTACGGTAGCTGGTACTC TCAATTACGTGTTCAAGGTCT 55.0 38.1 58.0 55.2 1227-1241 bp 1.0 49.5
psbJ-petA_p2 GGTACGGTAGCTGGTACTCT TCAATTACGTGTTCAAGGTCT 55.0 38.1 58.0 55.2 1226-1240 bp 1.0 49.5
psbJ-petA_p3 ACGGTAGCTGGTACTCTTGT TCAATTACGTGTTCAAGGTCT 50.0 38.1 58.1 55.2 1223-1237 bp 1.0 49.3
psbJ-petA_p4 AGGTACGGTAGCTGGTACTC TGCATCTGTTATCTTAGCACA 55.0 38.1 58.0 55.0 1194-1208 bp 1.0 48.3
psbJ-petA_p5 GGTACGGTAGCTGGTACTCT TGCATCTGTTATCTTAGCACA 55.0 38.1 58.0 55.0 1193-1207 bp 1.0 48.3