Hotspot

petL-psbE

Genome · 17587-18339 · 753 bp

Back to Ulota

Pi 0.00399
MarkerSeek score 61.20
Primer available yes
Label petL-psbE

Feature Metadata

label_name
petL-psbE
feature_type
igs
region
Genome
length_bp
753
variable_sites
3
pi
0.00399
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petL-psbE_p1 AGCTAAAAACAAAGAGGCAAACA CAAAAGGGCGTTCTCCTGTA 34.8 50.0 57.7 57.8 837-838 bp 1.0 52.3
petL-psbE_p2 AGCTAAAAACAAAGAGGCAAACA GGGCGTTCTCCTGTATTTCC 34.8 55.0 57.7 58.3 832-833 bp 1.0 51.0
petL-psbE_p3 AGCTAAAAACAAAGAGGCAAACA GGCGTTCTCCTGTATTTCCA 34.8 50.0 57.7 57.6 831-832 bp 1.0 50.5
petL-psbE_p4 AGCTAAAAACAAAGAGGCAAACA TATCAGCAAAAGGGCGTTCT 34.8 45.0 57.7 57.5 843-844 bp 1.0 49.8
petL-psbE_p5 GCTAAAAACAAAGAGGCAAACA CAAAAGGGCGTTCTCCTGTA 36.4 50.0 56.3 57.8 836-837 bp 1.0 49.1