Hotspot

ycf45

LSC · 60402-61748 · 1347 bp

Back to Ulnaria

Pi 0.0861

MarkerSeek score 77.30

Primer available yes

Label ycf45

Feature Metadata

label_name: ycf45
feature_type: gene
region: LSC
length_bp: 1347
variable_sites: 116
pi: 0.0861
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf45_p1	ACGTCGACATGCGTATAAGG	GATTTGAACCTACGACCCCC	50.0	55.0	57.9	58.0	2210 bp	0.5	58.8
ycf45_p2	ACGTCGACATGCGTATAAGG	GGATTTGAACCTACGACCCC	50.0	55.0	57.9	58.0	2211 bp	0.5	58.8
ycf45_p3	AAAAACAAGGAAAGCCGCAG	GATTTGAACCTACGACCCCC	45.0	55.0	57.8	58.0	2267 bp	0.5	57.9
ycf45_p4	AAAAACAAGGAAAGCCGCAG	GGATTTGAACCTACGACCCC	45.0	55.0	57.8	58.0	2268 bp	0.5	57.9
ycf45_p5	ACGTCGACATGCGTATAAGG	GGTTTTGGGGAATCACCGAT	50.0	50.0	57.9	58.2	2573 bp	0.5	57.9