Hotspot

ycf35

SSC · 99790-100158 · 369 bp

Back to Ulnaria

Pi 0.0954

MarkerSeek score 74.50

Primer available yes

Label ycf35

Feature Metadata

label_name: ycf35
feature_type: gene
region: SSC
length_bp: 369
variable_sites: 35
pi: 0.0954
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf35_p1	AGAGATGGTCGCCCTATTGA	TGGGCATTTGGTTAACAGCT	50.0	45.0	57.9	58.0	1240-1249 bp	1.0	88.1
ycf35_p2	AGAGATGGTCGCCCTATTGA	ATGGGCTGACATCATCAACC	50.0	50.0	57.9	57.9	1134-1143 bp	1.0	87.8
ycf35_p3	AGAGATGGTCGCCCTATTGA	TTCTCAGCTATGCACGGTTC	50.0	50.0	57.9	58.0	1451-1460 bp	1.0	87.5
ycf35_p4	AGAGATGGTCGCCCTATTGA	CTCAGCTATGCACGGTTCTT	50.0	50.0	57.9	58.0	1449-1458 bp	1.0	87.5
ycf35_p5	AGAGATGGTCGCCCTATTGA	AAGTCCCTCCGTTCCCAATA	50.0	50.0	57.9	58.0	794 bp	0.5	59.0