Hotspot

rpoB

LSC · 36099-40235 · 4137 bp

Back to Ulnaria

Pi 0.0728

MarkerSeek score 71.70

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 4137
variable_sites: 301
pi: 0.0728
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	ACCATGTACGCTAGCAGTTG	ACCAAATCGAATCCAAGATCCA	50.0	40.9	58.0	57.8	1153 bp	1.0	69.3
rpoB_p2	CACCATGTACGCTAGCAGTT	ACCAAATCGAATCCAAGATCCA	50.0	40.9	58.0	57.8	1154 bp	1.0	69.2
rpoB_p3	TGCAGGACAAGCTTCCATTT	ACCAAATCGAATCCAAGATCCA	45.0	40.9	58.0	57.8	850 bp	0.5	42.1
rpoB_p4	TTACCTGCAGGACAAGCTTC	ACCAAATCGAATCCAAGATCCA	50.0	40.9	57.8	57.8	855 bp	0.5	40.6
rpoB_p5	AAACCACCATGTACGCTAGC	ACCAAATCGAATCCAAGATCCA	50.0	40.9	58.3	57.8	1158 bp	0.5	40.0