Hotspot

ftsH

LSC · 56821-58692 · 1872 bp

Back to Ulnaria

Pi 0.078

MarkerSeek score 75.30

Primer available yes

Label ftsH

Feature Metadata

label_name: ftsH
feature_type: gene
region: LSC
length_bp: 1872
variable_sites: 146
pi: 0.078
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ftsH_p1	TCCTGAAGGTGGTCGTTACT	CCATTTTGATAGCGAGTGGC	50.0	50.0	58.0	57.2	2968 bp	0.5	53.9
ftsH_p2	TCCTGAAGGTGGTCGTTACT	CGCAAGTACTTGTGCAGACA	50.0	50.0	58.0	58.8	2906 bp	0.5	53.7
ftsH_p3	AACGCGTTTGATGCTAGCTA	CGAACAACAGCAGGATAACG	45.0	50.0	58.0	57.2	2650 bp	0.5	53.7
ftsH_p4	TAACGCGTTTGATGCTAGCT	CGAACAACAGCAGGATAACG	45.0	50.0	58.0	57.2	2651 bp	0.5	53.7
ftsH_p5	GGGCAATGAACGTTGAATGG	CGAACAACAGCAGGATAACG	50.0	50.0	58.0	57.2	2805 bp	0.5	53.7