Hotspot

ccs1

SSC · 101622-102887 · 1266 bp

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Pi 0.1

MarkerSeek score 78.80

Primer available yes

Label ccs1

Feature Metadata

label_name: ccs1
feature_type: gene
region: SSC
length_bp: 1266
variable_sites: 127
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccs1_p1	ATTCCCATTCACGACCCATG	AGGCCGTTTTGATGAAGTCT	50.0	45.0	57.9	57.4	2116 bp	1.0	81.3
ccs1_p2	ATGTTGTGCTCTGCTTGGAA	AGGCCGTTTTGATGAAGTCT	45.0	45.0	58.0	57.4	2294 bp	1.0	81.2
ccs1_p3	TGTTGTGCTCTGCTTGGAAT	AGGCCGTTTTGATGAAGTCT	45.0	45.0	58.0	57.4	2293 bp	1.0	81.2
ccs1_p4	GAACCGTGCATAGCTGAGAA	AGGCCGTTTTGATGAAGTCT	50.0	45.0	58.0	57.4	2369 bp	1.0	81.0
ccs1_p5	AAGAACCGTGCATAGCTGAG	AGGCCGTTTTGATGAAGTCT	50.0	45.0	58.0	57.4	2371 bp	1.0	81.0