Hotspot

trnI(GAT)-rns

IRa · 113039-113094 · 56 bp

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Pi 0.0545

MarkerSeek score 80.50

Primer available yes

Label trnI(GAT)-rns

Feature Metadata

label_name: trnI(GAT)-rns
feature_type: igs
region: IRa
length_bp: 56
variable_sites: 3
pi: 0.0545
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI(GAT)-rns_p1	TACCAGCTGAGCTATACCCC	GACTGGGGTGAAGTCGTAAC	55.0	55.0	58.0	57.9	208-209 bp	1.0	80.8
trnI(GAT)-rns_p2	ATTTGAACCAGAGACCTCGC	GACTGGGGTGAAGTCGTAAC	50.0	55.0	57.9	57.9	172-173 bp	1.0	80.4
trnI(GAT)-rns_p3	CCTTGGGCCATTCTGGATTT	GACTGGGGTGAAGTCGTAAC	50.0	55.0	58.1	57.9	188-189 bp	1.0	80.1
trnI(GAT)-rns_p4	TACCAGCTGAGCTATACCCC	GGGGTGAAGTCGTAACAAGG	55.0	55.0	58.0	58.2	204-205 bp	1.0	79.9
trnI(GAT)-rns_p5	TACCAGCTGAGCTATACCCC	TGCCCGAAGTCGTTACTCTA	55.0	50.0	58.0	58.2	263-264 bp	1.0	79.7