Hotspot

rns-trnI(GAT)

IRb · 64881-64936 · 56 bp

Back to Ulnaria

Pi 0.0545

MarkerSeek score 80.50

Primer available yes

Label rns-trnI(GAT)

Feature Metadata

label_name: rns-trnI(GAT)
feature_type: igs
region: IRb
length_bp: 56
variable_sites: 3
pi: 0.0545
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-trnI(GAT)_p1	GACTGGGGTGAAGTCGTAAC	TACCAGCTGAGCTATACCCC	55.0	55.0	57.9	58.0	208-209 bp	1.0	80.8
rns-trnI(GAT)_p2	GACTGGGGTGAAGTCGTAAC	ATTTGAACCAGAGACCTCGC	55.0	50.0	57.9	57.9	172-173 bp	1.0	80.4
rns-trnI(GAT)_p3	GACTGGGGTGAAGTCGTAAC	CCTTGGGCCATTCTGGATTT	55.0	50.0	57.9	58.1	188-189 bp	1.0	80.1
rns-trnI(GAT)_p4	GGGGTGAAGTCGTAACAAGG	TACCAGCTGAGCTATACCCC	55.0	55.0	58.2	58.0	204-205 bp	1.0	79.9
rns-trnI(GAT)_p5	TGCCCGAAGTCGTTACTCTA	TACCAGCTGAGCTATACCCC	50.0	55.0	58.2	58.0	263-264 bp	1.0	79.7