Hotspot

rpl2

IRa · 157543-159052 · 1510 bp

Back to Ulmus

Pi 0.000853
MarkerSeek score 36.90
Primer available yes
Label rpl2

Feature Metadata

label_name
rpl2
feature_type
gene
region
IRa
length_bp
1510
variable_sites
4
pi
0.000853
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2_p1 CGCTTCAACCGGGTTATTCT TTTTAGTGAACGTGGCACAA 50.0 40.0 58.3 56.1 1676-1698 bp 1.0 63.3
rpl2_p2 CGCTTCAACCGGGTTATTCT TTTTTAGTGAACGTGGCACA 50.0 40.0 58.3 56.1 1677-1699 bp 1.0 63.3
rpl2_p3 ATCATTACGCTTCAACCGGG TTTTAGTGAACGTGGCACAA 50.0 40.0 58.3 56.1 1683-1705 bp 1.0 62.7
rpl2_p4 ATCATTACGCTTCAACCGGG TTTTTAGTGAACGTGGCACA 50.0 40.0 58.3 56.1 1684-1706 bp 1.0 62.7
rpl2_p5 CGCTTCAACCGGGTTATTCT AGTGAACGTGGCACAATAAA 50.0 40.0 58.3 55.9 1672-1694 bp 1.0 61.4