Hotspot

ndhA

SSC · 124200-126754 · 2555 bp

Pi 0.0118

MarkerSeek score 70.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2555
variable_sites: 139
pi: 0.0118
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCATAATTCATGAACCCAGT	GCCCGCCAGGTTTTATCAAT	39.1	50.0	57.8	58.9	2769-2932 bp	1.0	55.4
ndhA_p2	TGACCATAATTCATGAACCCAGT	GCCCGCCAGGTTTTATCAA	39.1	52.6	57.8	58.4	2769-2932 bp	1.0	51.1
ndhA_p3	TGACCATAATTCATGAACCCAGT	TGGGAGAAGTTGATCGTTGA	39.1	45.0	57.8	56.5	2670-2833 bp	1.0	50.5
ndhA_p4	TGACCATAATTCATGAACCCAG	GCCCGCCAGGTTTTATCAAT	40.9	50.0	56.1	58.9	2769-2932 bp	1.0	49.9
ndhA_p5	GACCATAATTCATGAACCCAGT	GCCCGCCAGGTTTTATCAAT	40.9	50.0	56.1	58.9	2768-2931 bp	1.0	49.9