Hotspot

trnW-CCA-trnP-UGG

LSC · 69736-69904 · 169 bp

Back to Ulmus

Pi 0.0281
MarkerSeek score 64.20
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
169
variable_sites
16
pi
0.0281
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCACGTTCT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 226-254 bp 1.0 88.3
trnW-CCA-trnP-UGG_p2 GTTTTGGAGACCCACGTTCT CACGGGTTCAAATCCTGTCA 50.0 50.0 58.0 58.1 224-252 bp 1.0 88.3
trnW-CCA-trnP-UGG_p3 GTTTTGGAGACCCACGTTCT TGTCACGGGTTCAAATCCTG 50.0 50.0 58.0 58.1 227-255 bp 1.0 88.3
trnW-CCA-trnP-UGG_p4 GTTTTGGAGACCCACGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 237-265 bp 1.0 88.1
trnW-CCA-trnP-UGG_p5 GTTTTGGAGACCCACGTTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 238-266 bp 1.0 88.1