Hotspot

psbE-petL

LSC · 67774-69163 · 1390 bp

Back to Ulmus

Pi 0.0172
MarkerSeek score 66.90
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1390
variable_sites
95
pi
0.0172
unique_haplotype_count
19
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC AGCTGCTAGTAGAAAACCGA 50.0 45.0 57.8 56.3 1409-1466 bp 1.0 74.3
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC AGCTGCTAGTAGAAAACCGA 55.0 45.0 58.2 56.3 1404-1461 bp 1.0 74.0
psbE-petL_p3 ACGAATAACCAACCCGCAAT AGCTGCTAGTAGAAAACCGA 45.0 45.0 58.2 56.3 1495-1552 bp 1.0 73.9
psbE-petL_p4 TGCTGACGAATAACCAACCC AGCTGCTAGTAGAAAACCGA 50.0 45.0 58.2 56.3 1500-1557 bp 1.0 73.8
psbE-petL_p5 CGAATAACCAACCCGCAATG AGCTGCTAGTAGAAAACCGA 50.0 45.0 57.8 56.3 1494-1551 bp 1.0 73.8