Hotspot

ndhC-trnV-UAC

LSC · 53928-54647 · 720 bp

Back to Ulmus

Pi 0.0219
MarkerSeek score 65.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
720
variable_sites
59
pi
0.0219
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.94
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCCAGAAAATATCATATTCGT GTTCGAGTCCGTATAGCCCT 34.8 55.0 56.1 58.7 760-848 bp 1.0 49.9
ndhC-trnV-UAC_p2 TGCCCAGAAAATATCATATTCGT AGAAGGTCTACGGTTCGAGT 34.8 50.0 56.1 57.8 772-860 bp 1.0 49.8
ndhC-trnV-UAC_p3 TGCCCAGAAAATATCATATTCGT GAAGGTCTACGGTTCGAGTC 34.8 55.0 56.1 57.5 771-859 bp 1.0 49.8
ndhC-trnV-UAC_p4 TGCCAAAATAGGAATAACACTTGA AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.8 57.8 808-896 bp 1.0 49.5
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAACACTTGAG AGAAGGTCTACGGTTCGAGT 36.0 50.0 57.8 57.8 808-896 bp 1.0 49.5