Hotspot

trnF-GAA-ndhJ

LSC · 51519-52241 · 723 bp

Back to Ulmus

Pi 0.00906
MarkerSeek score 62.00
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
723
variable_sites
26
pi
0.00906
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA TGGATAGGATGGCCTTTACG 55.0 50.0 58.0 56.8 846-907 bp 1.0 76.6
trnF-GAA-ndhJ_p2 GGGATAGCTCAGCTGGTAGA GGATAGGATGGCCTTTACGT 55.0 50.0 58.0 56.8 845-906 bp 1.0 76.6
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA TGGATAGGATGGCCTTTACG 50.0 50.0 58.1 56.8 810-871 bp 1.0 76.3
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA GGATAGGATGGCCTTTACGT 50.0 50.0 58.1 56.8 809-870 bp 1.0 76.3
trnF-GAA-ndhJ_p5 GGGATAGCTCAGCTGGTAGA TGAAAGTTGGATAGGATGGCC 55.0 47.6 58.0 57.7 853-914 bp 1.0 76.0