Hotspot

rps2-rpoC2

LSC · 17582-17796 · 215 bp

Back to Ulmus

Pi 0.0211
MarkerSeek score 64.10
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
215
variable_sites
21
pi
0.0211
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 GGTGCCATTCTAGGATTCCA ACATCAGAACAATCATTTCTGGG 50.0 39.1 57.0 57.1 349-370 bp 0.956522 47.9
rps2-rpoC2_p2 ACTCCTGCTTCCATCATCTCT ACATCAGAACAATCATTTCTGGG 47.6 39.1 58.2 57.1 304-325 bp 0.956522 47.9
rps2-rpoC2_p3 TGAACTCCTGCTTCCATCATC ACATCAGAACAATCATTTCTGGG 47.6 39.1 57.7 57.1 307-328 bp 0.956522 47.8
rps2-rpoC2_p4 TGACCAAAATGAACTCCTGCT ACATCAGAACAATCATTTCTGGG 42.9 39.1 57.8 57.1 316-337 bp 0.956522 47.7
rps2-rpoC2_p5 GGTGCCATTCTAGGATTCCA CATCAGAACAATCATTTCTGGGA 50.0 39.1 57.0 56.8 348-369 bp 0.956522 47.4