Hotspot

trnS-GCU-trnG-GCC

LSC · 9122-10061 · 940 bp

Back to Ulmus

Pi 0.0672
MarkerSeek score 81.80
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
940
variable_sites
179
pi
0.0672
unique_haplotype_count
19
primer_available
yes
alignment_reliability
0.81
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA TAATCAAACCGAGGGATCCC 50.0 50.0 58.0 56.7 905-1220 bp 1.0 81.4
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC TAATCAAACCGAGGGATCCC 50.0 50.0 58.0 56.7 904-1219 bp 1.0 81.4
trnS-GCU-trnG-GCC_p3 ACGGAAAGAGAGGGATTCGA TAATCAAACCGAGGGATCCC 50.0 50.0 58.2 56.7 959-1274 bp 1.0 80.3
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA TTAATCAAACCGAGGGATCCC 50.0 47.6 58.0 57.4 906-1221 bp 1.0 79.4
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC TTAATCAAACCGAGGGATCCC 50.0 47.6 58.0 57.4 905-1220 bp 1.0 79.4