Hotspot

psbI-trnS-GCU

LSC · 8890-9033 · 144 bp

Back to Ulmus

Pi 0.0257
MarkerSeek score 66.80
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
144
variable_sites
19
pi
0.0257
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 ATGATCCGGGTCGTAATCCT TGGACTAAAGCGTCGGATTG 50.0 50.0 58.0 58.0 245-279 bp 1.0 89.5
psbI-trnS-GCU_p2 ATGATCCGGGTCGTAATCCT GTGGACTAAAGCGTCGGATT 50.0 50.0 58.0 58.0 246-280 bp 1.0 89.4
psbI-trnS-GCU_p3 ATGATCCGGGTCGTAATCCT GACTAAAGCGTCGGATTGCT 50.0 50.0 58.0 58.4 243-277 bp 1.0 86.7
psbI-trnS-GCU_p4 ATGATCCGGGTCGTAATCCT CTGAGTGGACTAAAGCGTCG 50.0 55.0 58.0 58.4 250-284 bp 1.0 86.3
psbI-trnS-GCU_p5 ATGATCCGGGTCGTAATCCT ATGGCTGAGTGGACTAAAGC 50.0 50.0 58.0 57.6 254-288 bp 1.0 85.9