Hotspot

trnH-GUG-psbA

LSC · 75-315 · 241 bp

Back to Ulmus

Pi 0.0581
MarkerSeek score 67.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
241
variable_sites
37
pi
0.0581
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.85
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 AATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 50.0 50.0 57.7 58.0 314-390 bp 1.0 89.1
trnH-GUG-psbA_p2 AATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.7 57.9 264-340 bp 1.0 88.8
trnH-GUG-psbA_p3 AATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.7 57.9 267-343 bp 1.0 88.7
trnH-GUG-psbA_p4 TTGCCTTAATCCACTTGGCT GCGCTAACCTTGGTATGGAA 45.0 50.0 57.4 58.0 321-397 bp 1.0 86.9
trnH-GUG-psbA_p5 TTGCCTTAATCCACTTGGCT CCTCTAGACCTAGCTGCTGT 45.0 55.0 57.4 57.9 271-347 bp 1.0 86.6