Hotspot

rps16

LSC · 5467-6841 · 1375 bp

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Pi 0.0292

MarkerSeek score 65.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1375
variable_sites: 40
pi: 0.0292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CACTGCCGTTTTGCATTTCA	ATCAATTCCATCCCGGTTCG	45.0	50.0	58.1	58.0	1466-1528 bp	1.0	80.9
rps16_p2	CACTGCCGTTTTGCATTTCA	CAATTCCATCCCGGTTCGAA	45.0	50.0	58.1	58.3	1464-1526 bp	1.0	79.2
rps16_p3	ACTGCCGTTTTGCATTTCAA	ATCAATTCCATCCCGGTTCG	40.0	50.0	57.4	58.0	1465-1527 bp	1.0	77.4
rps16_p4	CACTGCCGTTTTGCATTTCA	AATTCCATCCCGGTTCGAAA	45.0	45.0	58.1	57.5	1463-1525 bp	1.0	77.2
rps16_p5	CACTGCCGTTTTGCATTTCA	TTCCATCCCGGTTCGAAATT	45.0	45.0	58.1	57.5	1461-1523 bp	1.0	77.2