Hotspot

trnI(GAU)-rrn16

IRa · 151228-151522 · 295 bp

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Pi 0.0373
MarkerSeek score 73.90
Primer available yes
Label trnI(GAU)-rrn16

Feature Metadata

label_name
trnI(GAU)-rrn16
feature_type
igs
region
IRa
length_bp
295
variable_sites
11
pi
0.0373
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI(GAU)-rrn16_p1 CTATCCATGTGGCTGAGAGC CAAGGTAGCCGTACTGGAAG 55.0 55.0 57.8 57.7 421 bp 1.0 78.4
trnI(GAU)-rrn16_p2 GGCACAACGACGCAATTATC CAAGGTAGCCGTACTGGAAG 50.0 55.0 58.2 57.7 389 bp 1.0 78.4
trnI(GAU)-rrn16_p3 CGCTGATGAGCACATTGAAC CAAGGTAGCCGTACTGGAAG 50.0 55.0 57.8 57.7 440 bp 1.0 78.3
trnI(GAU)-rrn16_p4 CTATCCATGTGGCTGAGAGC AGTGACTGGAGTGAAGTCGT 55.0 50.0 57.8 58.3 443 bp 1.0 78.2
trnI(GAU)-rrn16_p5 CGCTGATGAGCACATTGAAC AGTGACTGGAGTGAAGTCGT 50.0 50.0 57.8 58.3 462 bp 1.0 78.1