Hotspot

rps15-ycf1

SSC · 137561-138421 · 861 bp

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Pi 0.13
MarkerSeek score 73.60
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
861
variable_sites
59
pi
0.13
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.53
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCTTTTTCTTCTTGTTCTTGTGA TGGTAGTCGTTTCAGTATGTCA 30.4 40.9 54.6 56.9 589-993 bp 1.0 50.0
rps15-ycf1_p2 TCTTCTTTTTCTTCTTGTTCTTGTGA TGGTAGTCGTTTCAGTATGTCA 30.8 40.9 57.7 56.9 592-996 bp 1.0 49.9
rps15-ycf1_p3 TCTTCTTTTTCTTCTTGTTCTTGTGA AGCTTGTATGAATCGGTATTGGT 30.8 39.1 57.7 58.0 628-1032 bp 1.0 49.7
rps15-ycf1_p4 TCTTTTTCTTCTTGTTCTTGTGA AGCTTGTATGAATCGGTATTGGT 30.4 39.1 54.6 58.0 625-1029 bp 1.0 49.7
rps15-ycf1_p5 CTTCTTTTTCTTCTTGTTCTTGTGA AGCTTGTATGAATCGGTATTGGT 32.0 39.1 56.4 58.0 627-1031 bp 1.0 49.7