Hotspot

ndhH-rps15

SSC · 137168-137281 · 114 bp

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Pi 0.0354

MarkerSeek score 68.30

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 114
variable_sites: 4
pi: 0.0354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	TCTTGTAGCCGGTACAGTCA	GCTGTTGGCGTATTTGTCAA	50.0	45.0	58.1	57.3	222-223 bp	1.0	75.5
ndhH-rps15_p2	AACACCATGCATTGATGGGT	GCTGTTGGCGTATTTGTCAA	45.0	45.0	58.1	57.3	276-277 bp	1.0	75.4
ndhH-rps15_p3	TCTTGTAGCCGGTACAGTCA	TTCTGGGAAAACGTCAACGG	50.0	50.0	58.1	58.7	241-242 bp	1.0	75.4
ndhH-rps15_p4	AACACCATGCATTGATGGGT	TTCTGGGAAAACGTCAACGG	45.0	50.0	58.1	58.7	295-296 bp	1.0	75.3
ndhH-rps15_p5	TCAGTCGAAGAACACCATGC	GCTGTTGGCGTATTTGTCAA	50.0	45.0	58.2	57.3	286-287 bp	1.0	74.2