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psaC-ndhE

SSC · 130245-131347 · 1103 bp

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Pi 0.0296

MarkerSeek score 58.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 1103
variable_sites: 18
pi: 0.0296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.55
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGCCCGTACACATTGAGTAC	CGTAACAGAAAATCAACGCGT	55.0	42.9	58.4	57.8	720-1214 bp	1.0	73.4
psaC-ndhE_p2	GCCCGTACACATTGAGTACA	CGTAACAGAAAATCAACGCGT	50.0	42.9	57.6	57.8	719-1213 bp	1.0	73.2
psaC-ndhE_p3	AGGCCCGTACACATTGAGTA	CGTAACAGAAAATCAACGCGT	50.0	42.9	58.4	57.8	721-1215 bp	1.0	72.7
psaC-ndhE_p4	GGCCCGTACACATTGAGTAC	TCGTAACAGAAAATCAACGCG	55.0	42.9	58.4	57.6	721-1215 bp	1.0	71.3
psaC-ndhE_p5	GCCCGTACACATTGAGTACA	TCGTAACAGAAAATCAACGCG	50.0	42.9	57.6	57.6	720-1214 bp	1.0	71.1