Hotspot

rrn16-trnI(GAU)

IRb · 109265-109559 · 295 bp

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Pi 0.0373

MarkerSeek score 73.90

Primer available yes

Label rrn16-trnI(GAU)

Feature Metadata

label_name: rrn16-trnI(GAU)
feature_type: igs
region: IRb
length_bp: 295
variable_sites: 11
pi: 0.0373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnI(GAU)_p1	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	55.0	55.0	57.7	57.8	421 bp	1.0	78.4
rrn16-trnI(GAU)_p2	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	55.0	50.0	57.7	58.2	389 bp	1.0	78.4
rrn16-trnI(GAU)_p3	CAAGGTAGCCGTACTGGAAG	CGCTGATGAGCACATTGAAC	55.0	50.0	57.7	57.8	440 bp	1.0	78.3
rrn16-trnI(GAU)_p4	AGTGACTGGAGTGAAGTCGT	CTATCCATGTGGCTGAGAGC	50.0	55.0	58.3	57.8	443 bp	1.0	78.2
rrn16-trnI(GAU)_p5	AGTGACTGGAGTGAAGTCGT	CGCTGATGAGCACATTGAAC	50.0	50.0	58.3	57.8	462 bp	1.0	78.1