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rpl22-rps19

LSC · 90445-90501 · 57 bp

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Pi 0.0351

MarkerSeek score 72.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 57
variable_sites: 2
pi: 0.0351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TCGTGCCTTGAAAACAGACA	GAGACATGCCCGAAACGATA	45.0	50.0	58.0	57.8	187 bp	1.0	78.9
rpl22-rps19_p2	CTTTGACTTCTGTACGCGGT	GAGACATGCCCGAAACGATA	50.0	50.0	58.2	57.8	146 bp	1.0	77.6
rpl22-rps19_p3	TTGACTTCTGTACGCGGTTT	GAGACATGCCCGAAACGATA	45.0	50.0	57.8	57.8	144 bp	1.0	77.5
rpl22-rps19_p4	TTTGACTTCTGTACGCGGTT	GAGACATGCCCGAAACGATA	45.0	50.0	57.8	57.8	145 bp	1.0	77.5
rpl22-rps19_p5	TCGTGCCTTGAAAACAGACA	AGACATGCCCGAAACGATAA	45.0	45.0	58.0	57.3	186 bp	1.0	75.1