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petG-trnW(CCA)

LSC · 72470-72591 · 122 bp

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Pi 0.0574

MarkerSeek score 79.90

Primer available yes

Label petG-trnW(CCA)

Feature Metadata

label_name: petG-trnW(CCA)
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 7
pi: 0.0574
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW(CCA)_p1	ACAATACAGACGCGGTGATC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	231 bp	1.0	81.7
petG-trnW(CCA)_p2	CAATACAGACGCGGTGATCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	230 bp	1.0	81.7
petG-trnW(CCA)_p3	ACAATACAGACGCGGTGATC	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	195 bp	1.0	81.5
petG-trnW(CCA)_p4	CAATACAGACGCGGTGATCA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	194 bp	1.0	81.5
petG-trnW(CCA)_p5	AATACAGACGCGGTGATCAG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	229 bp	1.0	80.6